Mechanically it'll be fine. I'd say the biggest issue is likely to be trying to keep the wheel tensioned properly in the future. You can remember the first one while you're building and truing, but a year from now, you may need to re-true the wheel and you'll forget.
Which spoke needs to be touched? Pluck them. Plonk, plonk, plonk, PLINK! That one is too tight! Loosen it, and now the true is way off. Loosen the ones around it. Now there's a hop in the wheel. 45 minutes into the job, you'll remember there's this one that's a different gauge. Fix that hop, and you're back to finding the other loose one. What could have been a 5 minute job now takes an hour.