HELL YES!
What you get from a bike store is service after the sale, and the knowledge that the bike was assembled by someone that does it for a living.
Would you expect your local Target/Wally World/Kmart to adjust the bike after you've ridden it awhile? Bike stores typically offer free tuneups because cables stretch causing braking and shifting irregularities, things loosen and generally need to be adjusted.
Support the local guys....buy from a LBS.
David