The title character in The 40 Year Old Virgin (the current #1 movie in the U.S.) rides a bike to work, owns no car, and has never learned to drive. It's a real commuter bike, too, outfitted with fenders, a headlight, a milk crate bungee-corded to a rack, and two handlebar-mounted mirrors. They show him mostly riding on the street, though there's also a scene where he rides on the sidewalk and then, with some difficulty, crosses several lanes of traffic to get to his destination. You also see such real-world details as him carrying a front tire into his workplace every day, and stuffing his pant cuffs in his socks before riding. At one point in the movie, he receives a new bike, some sort of Trek mountain bike with a huge shock absorber where part of the frame should be. Though not as practical for commuting as the bike he already had, he is happy to receive it. (Go, product placement!)
But this is a mixed blessing. Although the character is endearing, he has many juvenille quirks--other than being a virgin, he also plays lots of video games and collects action figures. His bike riding is portrayed as just another sign of his refusal to grow up. At one point, another character says he gave up bike riding when he was six years old. The main character's car-free lifestyle is also shown to be a serious impediment to getting laid (a central goal during the film). On the other hand, at one scene in the end of the movie his bike gives him a unique advantage over cars.