All Bikes All The Time
Join Date: Feb 2005
Location: Boise, ID
Bikes: Giant TCR 0, Lemond Zurich, Giant NRS 1, Jamis Explorer Beater/Commuter, Peugeot converted single speed
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The fact that the store is a big chain actually serves to decrease your chances of successfully negotiating a better price, imo. There is very little local pricing autonomy (ability to think and act for oneself) afforded to local managers of chain stores. I doubt you will have any luck at all if it is an REI or something.
That being said, $50 off a $400 bike is not unheard of and if the entire chain is local, that might be entirely different.