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Thread: need help

  1. #1
    AEO
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    need help

    How do I design a super bug that will wipe out all celery and shiitake mushrooms?
    Food for thought: if you aren't dead by 2050, you and your entire family will be within a few years from starvation. Now that is a cruel gift to leave for your offspring. ;)
    http://sanfrancisco.ibtimes.com/arti...ger-photos.htm

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    Senior Member DannoXYZ's Avatar
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    No way, I like celery and shiitake mushroom. We need a genetically-engineered bacteria that makes cocaine!!!

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    Gears? CliftonGK1's Avatar
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    Specific to just those two items? That is what would make it difficult...

    First you need to isolate a targetted activation sequence specific to those two organisms and not shared by anything else (or else you end up with cross-contamination, which is likely to happen through general mutation anyhow.)
    Then you need to design your apoptotic method; i.e. How are the cells going to die? Is this superbug going to work as a competitor for intracellular resources? Is it going to release a competitive binding agent and block metabolic pathways?
    You need a genomic sequence and a vector. A plasmid sequence is pretty simple to build with the right restriction enzymes; it's a benchtop reaction. The selection for the vector isn't too difficult. You want something wtih reasonable transmissability between organisms and a high replication rate. From there, it's a matter of transfecting your sequence into the vector (I suggest electroporation) and unleashing a wave of doom.
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    Celery is the perfect foil to the 'excessive heat syndrome' experienced by wimps who can't handle good buffalo wings, so it does have a use in society; the mealy little screams get on the nerves, y'know?

    Shiitake mushrooms bailed Letterman out of a bad interview with Steven Seagal.

  5. #5
    AEO
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    Quote Originally Posted by DX-MAN View Post
    Celery is the perfect foil to the 'excessive heat syndrome' experienced by wimps who can't handle good buffalo wings, so it does have a use in society; the mealy little screams get on the nerves, y'know?

    Shiitake mushrooms bailed Letterman out of a bad interview with Steven Seagal.
    celery would foil my plans to eat the hot wings in the first place.
    Food for thought: if you aren't dead by 2050, you and your entire family will be within a few years from starvation. Now that is a cruel gift to leave for your offspring. ;)
    http://sanfrancisco.ibtimes.com/arti...ger-photos.htm

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    AEO
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    Quote Originally Posted by CliftonGK1 View Post
    Specific to just those two items? That is what would make it difficult...

    First you need to isolate a targetted activation sequence specific to those two organisms and not shared by anything else (or else you end up with cross-contamination, which is likely to happen through general mutation anyhow.)
    Then you need to design your apoptotic method; i.e. How are the cells going to die? Is this superbug going to work as a competitor for intracellular resources? Is it going to release a competitive binding agent and block metabolic pathways?
    You need a genomic sequence and a vector. A plasmid sequence is pretty simple to build with the right restriction enzymes; it's a benchtop reaction. The selection for the vector isn't too difficult. You want something wtih reasonable transmissability between organisms and a high replication rate. From there, it's a matter of transfecting your sequence into the vector (I suggest electroporation) and unleashing a wave of doom.
    Have fun!
    sounds like a plan, now all I need is atomic bacteria, any idea where I can get some?
    Food for thought: if you aren't dead by 2050, you and your entire family will be within a few years from starvation. Now that is a cruel gift to leave for your offspring. ;)
    http://sanfrancisco.ibtimes.com/arti...ger-photos.htm

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    Ogr8nwmypstmksnosnse pgoat's Avatar
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    maybe he can help

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    Old Fogy
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    Misanthrope!

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    Hey guyz? Guyz? Wait up!! Siu Blue Wind's Avatar
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    Why are you messin' with me and my means of survival?
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    Quote Originally Posted by AEO View Post
    sounds like a plan, now all I need is atomic bacteria, any idea where I can get some?

    Hang around the effluent discharge outlet at your local bad tex/mex eatery. That stuff is so atomic it's radioactive.

    All you'd need to do is spray it on the offending celery or mushroom crops, report it to the FDA and you're done. (hey, it worked for lettuce a couple years ago)
    I am Fred, hear me slurp my Grande Mocha.

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    bread it the foil to excessive heat syndrome, not celery. freaking amateurs.
    sigless at the moment....

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    Quote Originally Posted by DX-MAN View Post
    Celery is the perfect foil to the 'excessive heat syndrome' experienced by wimps who can't handle good buffalo wings, so it does have a use in society; the mealy little screams get on the nerves, y'know?

    Shiitake mushrooms bailed Letterman out of a bad interview with Steven Seagal.

    no no no...the Celery is there as a natural dental floss to get the crud out of your teeth (or tooth, as the case may be).

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    Celery is the modern diet-pill for sorority chics...

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