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Old 09-04-10, 12:13 PM   #1
AEO
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need help

How do I design a super bug that will wipe out all celery and shiitake mushrooms?
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Food for thought: if you aren't dead by 2050, you and your entire family will be within a few years from starvation. Now that is a cruel gift to leave for your offspring. ;)
http://sanfrancisco.ibtimes.com/arti...ger-photos.htm
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Old 09-04-10, 01:29 PM   #2
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No way, I like celery and shiitake mushroom. We need a genetically-engineered bacteria that makes cocaine!!!
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Old 09-04-10, 03:14 PM   #3
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Specific to just those two items? That is what would make it difficult...

First you need to isolate a targetted activation sequence specific to those two organisms and not shared by anything else (or else you end up with cross-contamination, which is likely to happen through general mutation anyhow.)
Then you need to design your apoptotic method; i.e. How are the cells going to die? Is this superbug going to work as a competitor for intracellular resources? Is it going to release a competitive binding agent and block metabolic pathways?
You need a genomic sequence and a vector. A plasmid sequence is pretty simple to build with the right restriction enzymes; it's a benchtop reaction. The selection for the vector isn't too difficult. You want something wtih reasonable transmissability between organisms and a high replication rate. From there, it's a matter of transfecting your sequence into the vector (I suggest electroporation) and unleashing a wave of doom.
Have fun!
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Old 09-04-10, 04:58 PM   #4
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Celery is the perfect foil to the 'excessive heat syndrome' experienced by wimps who can't handle good buffalo wings, so it does have a use in society; the mealy little screams get on the nerves, y'know?

Shiitake mushrooms bailed Letterman out of a bad interview with Steven Seagal.
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Old 09-04-10, 06:07 PM   #5
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Celery is the perfect foil to the 'excessive heat syndrome' experienced by wimps who can't handle good buffalo wings, so it does have a use in society; the mealy little screams get on the nerves, y'know?

Shiitake mushrooms bailed Letterman out of a bad interview with Steven Seagal.
celery would foil my plans to eat the hot wings in the first place.
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Food for thought: if you aren't dead by 2050, you and your entire family will be within a few years from starvation. Now that is a cruel gift to leave for your offspring. ;)
http://sanfrancisco.ibtimes.com/arti...ger-photos.htm
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Old 09-04-10, 06:08 PM   #6
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Specific to just those two items? That is what would make it difficult...

First you need to isolate a targetted activation sequence specific to those two organisms and not shared by anything else (or else you end up with cross-contamination, which is likely to happen through general mutation anyhow.)
Then you need to design your apoptotic method; i.e. How are the cells going to die? Is this superbug going to work as a competitor for intracellular resources? Is it going to release a competitive binding agent and block metabolic pathways?
You need a genomic sequence and a vector. A plasmid sequence is pretty simple to build with the right restriction enzymes; it's a benchtop reaction. The selection for the vector isn't too difficult. You want something wtih reasonable transmissability between organisms and a high replication rate. From there, it's a matter of transfecting your sequence into the vector (I suggest electroporation) and unleashing a wave of doom.
Have fun!
sounds like a plan, now all I need is atomic bacteria, any idea where I can get some?
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Food for thought: if you aren't dead by 2050, you and your entire family will be within a few years from starvation. Now that is a cruel gift to leave for your offspring. ;)
http://sanfrancisco.ibtimes.com/arti...ger-photos.htm
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Old 09-04-10, 06:13 PM   #7
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maybe he can help

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Old 09-04-10, 08:51 PM   #8
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Misanthrope!
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Old 09-04-10, 08:51 PM   #9
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Why are you messin' with me and my means of survival?
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Old 09-05-10, 11:43 AM   #10
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sounds like a plan, now all I need is atomic bacteria, any idea where I can get some?

Hang around the effluent discharge outlet at your local bad tex/mex eatery. That stuff is so atomic it's radioactive.

All you'd need to do is spray it on the offending celery or mushroom crops, report it to the FDA and you're done. (hey, it worked for lettuce a couple years ago)
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Old 09-05-10, 12:32 PM   #11
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bread it the foil to excessive heat syndrome, not celery. freaking amateurs.
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Old 09-05-10, 02:14 PM   #12
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Originally Posted by DX-MAN View Post
Celery is the perfect foil to the 'excessive heat syndrome' experienced by wimps who can't handle good buffalo wings, so it does have a use in society; the mealy little screams get on the nerves, y'know?

Shiitake mushrooms bailed Letterman out of a bad interview with Steven Seagal.

no no no...the Celery is there as a natural dental floss to get the crud out of your teeth (or tooth, as the case may be).
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Old 09-06-10, 12:12 PM   #13
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Celery is the modern diet-pill for sorority chics...
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