Join Date: Aug 2003
Bikes: '03 Fuji Roubaix Pro; '03 KleinGi Attitude; '06 Soma Rush; '04 Surly Cross-Check; '06 Soma Rush; '07 Scott CR1 / Chorus
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A difference, but not much difference. Think about how much easier your current 25 is to the 23 - the 27 will be easier again by about the same. 21-23-25 gets replaced by 21-24-27.
What crankset are you currently running? If it's a double and you need more climbing gears, you'll probably be better off looking at a compact crankset.