I rode 143 miles about a month ago. I posted about it here. I washed my clothes two days before my ride with a new soap and it was concentrated. It did not rinse out. I posted a picture of my bike at the laundromat in the setting sun, rode home and hung my clothes to dry.
This mile long roundtrip was the only shakedown ride I took before my tour. My bike wasn't set up perfect and I had some problems, but nothing serious. The next morning I put on a pair of soapy shorts and wore them until midnight. I met up with some friends for drinks after work in Northern Virginia. I didn't realize why my ass was raw. I told my friends I was planing to ride 150 miles the next day, but I didn't tell them my about my sore butt.
The next morning, I pulled on another pair of soapy shorts and proceeded to ride 143 miles, a bit short of my goal. There was chafing, something I'd never experienced before. The soap wore a couple of holes in my skin. They didn't bleed but pus came out and scabs formed.
A week later I hit my tailbone really hard on my saddle on a failed attempt to gain a curb. It was painful for three week and I didn't want to ride, but it feels much better now. In the meantime I hurt my knees and back riding out of the saddle (for obvious reason), but they are felling better now.
I want to do 150 and maybe a double century. I'm feeling better and my shorts are rinsed out.