I remember Brandt's dis on plucking. I also remember watching a good violinist playing for an oscilloscope. What sounded like a clean tone coming from a musical instrument looked exactly like Brandt's description of the spoke. I figure if a human can pick out the pitch of a violin, guitar, etc. and tune to that, a trained human ear can listen to the sound of a spoke and tune that as well.
These electronic tuners have to deal with all the cross-talk from other strings, so I'd expect they can do the same with a wheel.