Darth Lefty 

Back when Stan's brought out the Dart they showed a guy using a cartridge on the trailside but their bumf said the sealant should be replaced once back home.

No reason given but I'd speculate that the CO2 is going to acidify the sealant when it goes into solution as carbonic acid and interfere with the latex inhibitor, which is alkaline.

I carry a pump on my tubeless bikes

Darth Lefty 

From Stan's website FAQ

rsbob 

Have a bottle of Silca’s Ultimate Sealant which I will be trying with the new rims/tires. Have used Stans and Orange which have been fine, but then I just don’t get punctures - knock on wood.

Iride01 

Is freezing a permanent issue for the sealant? CO2 isn't any colder than O2 or air when allowed to come to ambient temperature. And inflators that give you finer control of how fast the CO2 flows lets you add it slowly to the tire so there is less cooling going on while Boyles Law is showing itself.

I guess this question sort of begs the question, do people ride tubeless tires in sub-freezing temps? I suspect they do.

DangerousDanR

My Fatbike is tubeless. It has been out in -20F. I had been riding the MTB as well to commute in sub-freezing. I believe the issue is the chemical interaction between the sealant and the CO2, not freezing.

Even with Orange Seal Sub-Zero sealant, my garage has dropped well below the freezing point of my sealant, and I have not noticed any issues.

Calsun

Sealants use air which is 78% nitrogen and 21% oxygen. So a puncture with CO2 might not be sealed with the tire sealant but this is a guess. It really does not matter as CO2 leaks much faster through tire tubes and so should be replaced with air at the first opportunity.

I go years without a puncture on the roads and so carrying a full length pump makes little sense when a CO2 cartride fits in the pouch with the spare tube under the seat.