32" inseam at your height seems long but then I am 5'6" and have short legs. Frame size of 53cm is a good starting point but that is all it is-A starting point.

I take it you have not ridden a Road bike for some length of time so do not know bike set up yet. The main point about that is that it "Must Fit"- obvious- but must be comfortable.

Top tube length is normally the measurement to go by as this is a fixed size on a bike. Standover height will not affect fit until you get off the saddle and stand up so is not that imortant. Saddle and bar position can be altered.

Choosing the right size bike is an art that takes several bikes to get right and is relatively easy. What is not is finding your Local Bike shop (LBS) that will give you all the help possible to get somewhere near the right bike first time out---So first find your LBS.

Sorry to throw a few more problems at you- but only one person can get the right bike for you-- and that is you-----But hopefully with the help of a good bike shop.

And I ride several bikes- all different sizes but they do fit me- Some fitted off the shelf and some had to have a few parts changed to get right. And you cannot rely on a 53cm frame fitting you as good as another manufacturers 53cm frame- you might need a 51 or even a 54.