Only place I've had a problem bringing my bike into hotel rooms has been in Las Vegas. There the ground floor is usually arranged so that you are forced to walk past crowded rows of slot machines to get to the elevators that lead to the hotel rooms and wheeling a bike through could pose a problem. But they were very accommodating with secure storage and quickly retrieving my bike when requested. At hotels in other cities I follow the 'don't ask/don't tell' policy, try to take my bike in and out through a side entrance when possible, and, of course, make sure the bike doesn't leave any marks or dirt in the room.