With my limited experience I think that the saddle does play a part in comfort but time in the saddle is the most important. When I first started road biking this year I could only go about 10 miles before my ass hurt so bad I had to stand or get off the bike. After riding about 500 miles I can ride for 35 miles straight without any problems at all. That is with the same saddle (Specialized Avitar BG) and the same shorts (Pear Izumi Elite) that I started with.