http://veloroutes.org
http://bikreroutestoaster.com
http://ridewithgps.com/
http://www.bikely.com/
http://bycycle.org/
San Francisco bike route planner
http://www.bikemetro.com/route/routehome.asp
http://www.radroutenplaner.nrw.de/RRP_home_02_en.html
Cycle Routes UK - Cycle Route Planner
Route Planning « Bike Michiana
http://toporoute.com/cgi-bin/bicycle.cgi
http://www.transportdirect.info/Web2/JourneyPlanning/FindCycleInput.aspx
http://www.cyclevancouver.ubc.ca/cv.aspx
http://efa.vvs.de/bike/XSLT_TRIP_REQUEST2?language=en
Routeslip.com
Cyclestreets
Bike the ****s Netherlands
GPS Routes UK


Some other route planners which may or may not work for bicycle routing:
RAC Route Planner
ViaMichelin
London Route Planner
Mappy Route Planner
AA Route Planner
Tom Tom Route Planner
Multimap Journey Planner
Green Flag
Mapquest Route Planner
Maporama Route Planner
Expedia Route Planner


and on and on

Personally, I prefer mapmyrides.com which has already been mentioned.
YMMV