Putting the 53t chainring on the inner position of a road double might give you chainstay clearance issues, depending on the frame.

But in general, the chainline of a road double (measured in between the two chainrings) is 43.5. Given a 5mm chainring spacing, this puts the inner chainring position at 41mm, which will line up with your track hubs 41mm standard chainline. Or it'll be close enough.