Originally Posted by ksisler

Looking at the pix of the chainstay with the tire in...it looks like the frame was build for very skinny tires and the current wheel has some fairly fat tires on it. That will make any issues with dish, spacers, etc., more likely to be noticed. I would suggesting borrowing a known good wheel from someone (or may from a LBS) and slide it all the way in, hold it there or tighten the QR, and see if the rim is centered between the stays or pretty close to centered. Also be sure to take out or replace that fender mount screw with a shorter one before you end up with the chain jammed in between it and the cog as that will not be a good situation. Of course, follow with all the other replies about spacers and whatnot...