chainline, assuming a properly aligned frame, is only contingent upon your hub, cog, bottom bracket, and cranks.

the only frame-related issues i've really run into are when the chainring/cranks bottom out on the seatstays. you tend to only really see this on frames wide rear spacing or when trying to pull in the chainline of certain road cranks. i'm assuming that your frame is designed with track/fixed in mind, so it should be fine.