Climbing elevation is the geography equivalent of calories burned. Lots of ways to come to an answer, but you need either a survey or a lab test for oxygen consumption to get even close to definitive.
That said, you should look at Delorme Topo if you want a big number. One route I looked at was about 100% over; it was a road built on an old railroad bed climbing a mountain, and I'm pretty sure there's no drop on that route. Topo interpolates between topo lines on the map, so its profile looked like a sawtooth.