Originally Posted by rpenmanparker

I am not entirely sure what you are asking, or what you mean by a "stand" (trainer? repair?) but shifting can be different on a mechanics stand than on the road and require a slight adjustment. If you are talking about two different rear wheels or wheel equivalents (a trainer setup of some sort), then absolutely yes. Shifting usually needs to be adjusted when changing wheels (or from a machine to a wheel) unless the hubs are identical. A change in position of the cassette relative to the derailleur of only 0.5 mm is still very significant as far as derailleur tuning is concerned involving several turns of the barrel adjuster.