I believe CO2 is typically stored in a compressed liquid state, while Nitrogen is stored in a compressed gaseous state.

So, while the compressed N2 may work, you get a lot of gas in very little space with the CO2.

It is also possible that your inflation system is not designed to take 2500 PSI. It will have flowing gas, but a pretty high spike in pressure.

I like to experiment some, but I'm not sure that is an experiment I'd choose to do. At least if I was to do it, I'd inspect the system carefully to determine whether it looks like it would blow. And, even so, wear safety gear for the first experiments.

Some people do use pure N2 in tires, but I believe generally filled out of large tanks.