Originally Posted by badger1

Yes.

I think this question comes up, in part, because of the association some see between a 'pad' (chamois, natural or artificial) and 'padding' in cycling shorts/bibs (aka 'Not Shorts' on Bike Forums).

To my mind, 'padding' is not the primary -- or even a secondary -- purpose of the chamois. Its primary purpose is to prevent chafing.

Wearing 'underwear' between skin and chamois defeats that purpose.

End serious response. Carry on.