My experience from back when I made the transition from a Specialized Crosstrail hybrid to a proper road bike was that the availability of more hand and riding positions was the biggest improvement to comfort. I would say not to expect a radical difference in the way the bike feels from a comfort perspective, but you'll be able to move from the hoods to the drops to the tops which will change the pressure you feel both in the saddle and in your hands. I found that with the hybrid, when I started to push 40-50 miles, I would start getting numbness in my hands. With the road bike, those small changes in position throughout the ride meant I never experienced the same as I progressed past 50 all the way to beyond 100 miles without any hand numbness. I'm sure it's different for everyone, but that was my experience.