I've never used it in a bicycle tire, but carried it when I rode motorcycles. While on a group ride, a fellow rider (crazy rider!) has a flat. We used my fix-a-flat. It worked for the rest of the ride (this rider rode FAST), so good thing it held. Don't know if he replaced the tube later or not (yes, it was a tubed tire). But it is not a permanent remedy--replace whatever you use it in asap.