It is possible, but not extremely likely, the frame is off to that degree. It can be check by a good shop, see Frame Alignmnet. The dropouts can be out of alignment, and this would make a wheel trickier to get in, and will stress the axle. See also Dropout Alignment

If you have a replaceable dropout, double check it is properly mounted. These can sometimes prevent a wheel from centering.

Be sure to double check the centering or dish of the rear wheel. See Wheel Dish