Pack three water bottles if it is hot. I use 2 with some type of electrolyte drink. One just water. Get way hydrated for 2 days-start tonight-feel like you have to pee when you start. Last year with the cold you did not need as much liquid and you had to pee all the time.
Avoid Coffee on Saturday. If you need caffine (like me) have a gel with caffine. I eat 4 or 5 Sushi type rolls at each non-neutral feed zones.
It's my secret weapon. Also if its hot have Thermotabs with you. They are a Salt Pill you buy at Smith's. You have to ask the pharmasist for them. One or two an hour after about 3 hours really helps. Also pack some type of antiinflamatory (Allieve, Ibeprohin) When I got to the top of Salt River Pass last year my knees were on fire-I had an Allieve Gel Cap and the pain vanished(until sunday morning)

It's a long ride with the first 100 miles mostly climbing so just pace yourself and don't overdo it trying to keep up with riders who are more powerful. Usually your starting group will just fly to Preston. If your in a pace group that you can keep up with try to stick with them. If your getting yo-yo'd, back off and wait for another pace line that is more your speed. So many riders go to hard and bonk in the first 100 miles. I could not believe in 2004 when we went via Soda Springs how many young studs flew by me in these 27 MPH pace lines only to see them bonking, struggling and hardly making into Montpielier. Stay within yourself and you will find when your leaving Star Valley that your strong, in a monster 2nd wind mode.

If you are not supported-stop and get liquid in Preston for sure-that's where the climbs start and if it's hot your going to suffer and not finish. It's all about finishing, it's all about finishing, it's all about finishing.
There is a neutral feed at Strawberry. If you still have one bottle left there it will get you to Montpeliear. They ususally pass out gels at Strawberry. Remember when you hit the feed zone at Strawberry(its a neutral zone so your sag will not be there) you still have to climb a few miles. Last year people got in real trouble at strawberry in that it was snowing, you were wet and it was cold. People hung around, in a daze there and proceded to get hypothermia. If they would have just fueled up and left, the climb would have immediatley warmed them up. Lot's of folks quit at Strawberry.
Fuel up and get gels, and bars or whatever suits you at Montpelier. Remember-it's all about finishing.

You should be able to Make it from Montpelier to Afton on three bottles, unless it's real hot. In that case you should stop at feed zone 4 and get liquid.
Once your in Afton make sure your with a group, pace line it all the way to Jackson and make sure you have lights in your SAG stuff for the Alpine stop. For many people (who don't eat sushi) your stomach starts to shut down around this time, especially if its hot, so a candy bar and a flat coke can really help-simple sugar will wake up your guts.

I've done LOTJA 2 times, last year ( I finished in the dark and rain) and the year before I made it in great shape. I honestly felt better after finishing LOTOJA, both times, than I did on most all of my training rides.
While your getting all your chains, cogs and stuff in order at the LBS, consider getting a Bento Bag (Contender Cycle) its a little nylon box
affair that straps to your top tube and you fill it with food things.
Remember-it'a all about-Nutrition, Hydration, Preparadness (cloths, gloves, lights, be ready for rain, snow, cold, wind and hot) and finishing. Ride your pace, watch your heart rate and you will smoke em.
Evaluate what your goals are. If you are competing to try to win your Category I don't have great advice. I'm not a good climber and my group will have to wait 1 or 2 minutes for me on the top of the passes. If you find yourself alone, no group to ride withyour can get in trouble, its a tough ride to not utilize drafting. If your goal is to finish (before dark) then don't be so hung up on not stopping. Take 5 to 8 minutes at the feed zones. Laugh and cry with your fellow riders and Have a Good Time-the best memories will be the people you encounter.
My bib is 5166, hope to see you on the road. If your a Cat 1 through Master-you better hope you don't see me on the road-because I am slow and you should be ahead of me.