Whatever hub you use, the rim centerline must wind up centered between the locknuts (the nuts on the inside of the dropouts). The cog on the drive side of a fixed gear or single speed hub makes the distance between the drive side flange and the centerline shorter than the distance between the non-drive side flange and the centerline, so the specific hub's drive side and non-drive side flanges must be measured relative to the locknuts on each end of the axle to determine the amount of dish.

Here's a drawing of a track chain line from Campagnolo Catalog 16 that may help.