No. There are no loose parts, no parts to lose. Removal of the bolt requires a 3/16" hex wrench, rather than a metric one. After breaking the frame into two pieces, I put the bolt back in its place and tighten it well, so I know the bolt will be there when I go to put things back together. My experience has been that anything you put in the case should be big enough that it is unlikely to be left out during the repack by security. So put any little stuff in a big Zip-lock or something similar. I try to use clear bags, thinking the more they can see, the less the need to take apart. Maybe not true, but I haven't lost any small tools or parts yet in six years of this.

If your frame is older and the hinge bolt has never been removed, it may be a challenge. My friend's was rusted and required a long soak with a penetrating solvent. WD wouldn't touch it. We used a CRC brand product in a spray can, called Knock'er Loose. There is a thread about this in the Folder forum. There are a bunch of such products available. I found it at a hardware store. Auto parts stores carry this sort of thing as well.