I just built my first wheel last night. A 600 hub laced with DT Swiss DB spokes to a Matrix Iso-CII Aero rim. It was actually really fun.
All I used was a spoke wrench, screwdriver and a flipped over bike. No truing stand. Turned out pretty darn well. Tension is pretty even, and just as high as my pro-built wheels.
Its laterally in true under 1mm. Radially it might be 1 off or so, but I'm working on that. Dishing wasnt nearly as hard as I figured it would be. I just eyed everything up.
Surprisingly, I brought it to my local wheel builder to check over, and he didnt even charge me saying it was good to go.
Thanks Sheldon Brown!