I am running a machine built velocity Deep V rear wheel on my fixie. There have been times when I got flat running a 28C tire that took awhile before realizing it went flat. The tread of the tire is pretty thick. The Deep V is a gold standard in durability. I think Jens5 wheelbuild components should give you what you want in terms of durability. Consider the purchase of Phil hubs to be the last.
If you don't want to ever think about losing a wheel again consider the Velocity Dyad 40 spoke laced to a Phil hub.