If you know that you are going to be wearing the tights the whole time of the ride then I prefer to have the chamois in them and ONLY wear the tights. Without the chamois is good for when your wearing them only to warm up and then you will take them off such as in a race situation. Having both knicks AND tights with a chamois is definitely too much and I find that when I wear non-padded tights over knicks that the tights move around too much for my liking.
Regards, Anthony