the chainline is determined by the HUB, not by the frame or by the spacing of the hub (unless you unevenly space the hub, which is not a done thing).

chainline is the measurement from the centerline of the hub to the center of the cog. changing how the frame holds the hub is not going to make a difference, except you can get the frame out of alignment.

measure from the center of your seatpost to the center of your chainring to determine how far off your chainline is. chances are your hub's chainline is 42.5mm. if you're within 2 or 3 millimeters of that at the chainring, you are fine.