Based on the mileage you state I would think about 3 weeks and the saddle will at least have started to break in, you'll feel the the indentations on the saddle, where your sit bone is constantly placed. The proofride is more of a waterproofing thing as opposed to a softener, so based on the 3 week breaking in period I would only apply proofride once and you've already done that. Over the course of a year I would only apply proofride maybe 3 or 4 times.