Originally Posted by
bagel007
Is it possible that while fixing the flat I accidently brought the wheel out of its delicate balance?
It's not delicate. Consider the hammering it receives when you ride it.
So, almost certainly not possible unless you jumped on it. Most likely you put it back in a little crooked. I guess there's an outside chance of some sort of weird interaction between tyre pressure and spoke tension going on, but highly doubtful that there'd be any difference between your spoke tension with an inflated tyre pre and post flat fix.