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#3426
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...something to do with the steerer. Maybe the super duty steerer tubing Schwinn insisted on speccing for a long time ?Thicker tubing, so took a smaller diameter stem. But that's only a guess. It might just be in there by mistake. I'm not one of the many Schwinn experts on Bikeforums. A few of them post exhaustively detailed explanations of Schwinn history, that tire me out just looking at them. I think whoever gave you that warning did you a big favor.
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#3428
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Apparently our Maryland office is snowed in, so they're all getting a Snow Day. We used to think that was unfair, till we had AQI bad enough to tell people not to come in. Of course, it's not like you can escape the smoky air by staying home, AND it's not like anyone growing cells didn't have to come to work to take care of them.
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#3429
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Emmitt wasn't that elusive in terms of breaking tackles, IMO. That wasn't his strong suit. He had great vision, great stamina and great determination. One unheralded skill he had was the ability to lean away from hard tackles when it was inevitable he couldn't avoid the tackle. He softened the blow by doing that, which in turn avoided injuries and lengthened his career, allowing him to pile up all those yards.
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#3430
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It may be a few months before I am within striking distance of bike PRs, but today I PR'd my walk to work at a bit under 15'. And I didn't even have a tailwind Velo Vol
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Their defense is confused. Can’t cover anyone, often leaving the middle of the field wide open. Teams finally figured that out. And their tackling is horrible. Trying to grab up high instead of hitting in the middle. That doesn’t work with the strong receivers of today’s game. Look at all the RAC yardage last night. On top of all that, they take bad tackling angles after a catch.
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Officially 3.5” of snow last night, ending a 715 day streak without a 1” or more snowfall. I took the day off.
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he's had some tough health concerns In the last couple years. He's gone from a 40 waist down to a 34 from this. I just bought him some new pants in 36 and if he lets go without a belt they slide right down. It makes me worry. But I can't force him to eat.
Last edited by ls01; 01-16-24 at 11:51 AM.
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...something to do with the steerer. Maybe the super duty steerer tubing Schwinn insisted on speccing for a long time ?Thicker tubing, so took a smaller diameter stem. But that's only a guess. It might just be in there by mistake. I'm not one of the many Schwinn experts on Bikeforums. A few of them post exhaustively detailed explanations of Schwinn history, that tire me out just looking at them. I think whoever gave you that warning did you a big favor.
#3436
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#3437
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#3438
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#3439
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--Roseanne Roseannadanna
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#3442
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Oh?
What care does a growing cell require?
Wind chill?
Nope.
Most of the movie is filmed in late winter (there's a Saint Patrick's Day scene), but in one of the scenes where he's running through the forest you can see small leaves on the trees.
Yes.
It may be a few months before I am within striking distance of bike PRs, but today I PR'd my walk to work at a bit under 15'. And I didn't even have a tailwind Velo Vol
Nope.
Most of the movie is filmed in late winter (there's a Saint Patrick's Day scene), but in one of the scenes where he's running through the forest you can see small leaves on the trees.
Yes.
#3443
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See, this is why we can't have nice things. - - smarkinson
Where else but the internet can a bunch of cyclists go and be the tough guy? - - jdon
#3444
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That depends on the cell. All of them require a fairly rich growth medium, as well as certain growth factors (mostly supplied by serum). All need a very narrow pH range, provided by buffers in the medium and particular level of CO2 in the incubator - which is generally at 37 C. Some cells only grow attached to a surface, so they need to be kept in treated dishes or flasks, while others grow in suspension. Some cells only grow if they're close enough together, but others won't tolerate crowding, so they need to be maintained within certain densities. Some cells depend on factors they themselves secrete, so when you replace the medium you have to add back some percentage of the old medium. Some cells depend on factors produced by different cells, so you have to supplement their medium with a small percentage of spent medium from those other cell.
All of them can deplete the nutrients and growth factors in the medium and/or acidify the medium with their metabolic byproducts, so the medium has to be replaced before that happens.
Oh, and all of this needs to be sterile, because the cells divide maybe every 20 hours, whereas bacteria in that medium will divide every 20 MINUTES and will overgrow the culture within less than a day.
All of them can deplete the nutrients and growth factors in the medium and/or acidify the medium with their metabolic byproducts, so the medium has to be replaced before that happens.
Oh, and all of this needs to be sterile, because the cells divide maybe every 20 hours, whereas bacteria in that medium will divide every 20 MINUTES and will overgrow the culture within less than a day.
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"Don't take life so serious-it ain't nohow permanent."
"Everybody's gotta be somewhere." - Eccles
"Don't take life so serious-it ain't nohow permanent."
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#3445
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Oh the joys of microbiology. I've ran some Kirby-Bauer/MIZ tests here in years past, testing the antimicrobial efficacy of nanosilver and ZnO formulations (vs nanosilver only). Outside of that my microbio is basically nothing. Zero direct cell line exp. But I can comment on how challenging it is to analyze nanomaterial physical properties when dispersed in culture media. It's a messy soup of a matrix for sure.
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"Your beauty is an aeroplane;
so high, my heart cannot bear the strain." -A.C. Jobim, Triste
"Your beauty is an aeroplane;
so high, my heart cannot bear the strain." -A.C. Jobim, Triste
#3446
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Oh the joys of microbiology. I've ran some Kirby-Bauer/MIZ tests here in years past, testing the antimicrobial efficacy of nanosilver and ZnO formulations (vs nanosilver only). Outside of that my microbio is basically nothing. Zero direct cell line exp. But I can comment on how challenging it is to analyze nanomaterial physical properties when dispersed in culture media. It's a messy soup of a matrix for sure.
#3447
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nice one. genejockey maybe you can throw that around at work?
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"Your beauty is an aeroplane;
so high, my heart cannot bear the strain." -A.C. Jobim, Triste
"Your beauty is an aeroplane;
so high, my heart cannot bear the strain." -A.C. Jobim, Triste
#3448
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That depends on the cell. All of them require a fairly rich growth medium, as well as certain growth factors (mostly supplied by serum). All need a very narrow pH range, provided by buffers in the medium and particular level of CO2 in the incubator - which is generally at 37 C. Some cells only grow attached to a surface, so they need to be kept in treated dishes or flasks, while others grow in suspension. Some cells only grow if they're close enough together, but others won't tolerate crowding, so they need to be maintained within certain densities. Some cells depend on factors they themselves secrete, so when you replace the medium you have to add back some percentage of the old medium. Some cells depend on factors produced by different cells, so you have to supplement their medium with a small percentage of spent medium from those other cell.
All of them can deplete the nutrients and growth factors in the medium and/or acidify the medium with their metabolic byproducts, so the medium has to be replaced before that happens.
Oh, and all of this needs to be sterile, because the cells divide maybe every 20 hours, whereas bacteria in that medium will divide every 20 MINUTES and will overgrow the culture within less than a day.
All of them can deplete the nutrients and growth factors in the medium and/or acidify the medium with their metabolic byproducts, so the medium has to be replaced before that happens.
Oh, and all of this needs to be sterile, because the cells divide maybe every 20 hours, whereas bacteria in that medium will divide every 20 MINUTES and will overgrow the culture within less than a day.
#3449
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Depends if their "bend and break" defense or their "tight defense" used vs Dallas in quarters 1-3, KC game and Detroit game#2 scheme is employed. Joe Barry their defensive coordinate defers to running a bend, don't break defense but Packers get ran off literally and figuratively when executed.
#3450
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